MARK2 Antibody - C-terminal region (OAAB17362)

Data Sheet
 
Product Number OAAB17362
Product Page www.avivasysbio.com/mark2-emk-antibody-c-terminal-region-oaab17362.html
Name MARK2 Antibody - C-terminal region (OAAB17362)
Molecular Weight 87911 Da
Isotype Ig
NCBI Gene Id 2011
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols EMK1, EMK-1, PAR-1, Par1b, Par-1b
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB17362
Additional Information Precautions: MARK2 (EMK) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU, and RAB11FIP2. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule- dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells
Application Info WB: 1:1000
IHC-P: 1:50~100
Immunogen This MARK2 (EMK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 600-630 amino acids from the C-terminal region of human MARK2 (EMK).
Additional Information Cellular Location: Cell membrane; Peripheral membrane protein. Cytoplasm. Note=Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm
Tissue Location: High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney
Uniprot ID Q7KZI7
Protein Name Serine/threonine-protein kinase MARK2
Protein Accession # NP_001034558.2
Gene Symbol MARK2
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human; Mouse; Rat
Image 1
Mouse Thymus
Western blot analysis of anti-EMK Antibody (C-term) (Cat.# OAAB17362) in mouse thymus tissue lysates (35ug/lane). EMK(arrow) was detected using the purified Pab.
Image 2
Ramos
EMK Antibody (V581) (Cat. # OAAB17362) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the EMK antibody detected the EMK protein (arrow).
Image 3
hepatocarcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
 

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