MST1 Antibody - C-terminal region (OAAB17310)

• Product Number: OAAB17310
• Name: MST1 Antibody - C-terminal region (OAAB17310)
• Molecular Weight: 55630 Da
• Isotype: Ig
• NCBI Gene Id: 6789
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: KRS2, MST1, YSK3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MST1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2 Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1 Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This MST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the C-terminal region of human MST1.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm
  Tissue Location: Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed
• Uniprot ID: Q13043
• Protein Name: Serine/threonine-protein kinase 4
• Sample Type Confirmation:

  STK4 is supported by BioGPS gene expression data to be expressed in HL60

• Protein Accession #: NP_006273.1
• Gene Symbol: STK4
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: HL-60
  
  Western blot analysis of anti-MST1 Pab (Cat. # OAAB17310) in HL60 cell lysate. MST1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 3: 293
  
  Western blot analysis of STK4 (arrow) using MST1 Antibody (C-term) (Cat.# OAAB17310).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK4 gene (Lane 2) (Origene Technologies).

  STK4 is supported by BioGPS gene expression data to be expressed in HL60