MAP3K8 Antibody - C-terminal region (OAAB17302)

• Product Number: OAAB17302
• Name: MAP3K8 Antibody - C-terminal region (OAAB17302)
• Molecular Weight: 52925 Da
• Isotype: Ig
• NCBI Gene Id: 1326
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: COT, EST, ESTF, TPL2, AURA2, MEKK8, Tpl-2, c-COT
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: COT (MAP3K8/MEKK8) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This COT (MAP3K8/MEKK8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-445 amino acids from the C-terminal region of human COT (MAP3K8/MEKK8).
• Additional Information: Cellular Location: Cytoplasm.
  Tissue Location: Expressed in several normal tissues and human tumor-derived cell lines
• Uniprot ID: P41279
• Protein Name: Mitogen-activated protein kinase kinase kinase 8
• Protein Accession #: NP_001231063.1
• Gene Symbol: MAP3K8
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: 293
  
  Western blot analysis of MEKK8 (arrow) using MEKK8 Antibody (C-term) (Cat.# OAAB17302). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K8 gene (Lane 2) (Origene Technologies).