RIPK2 Antibody - C-terminal region (OAAB17272)

• Product Number: OAAB17272
• Name: RIPK2 Antibody - C-terminal region (OAAB17272)
• Molecular Weight: 61195 Da
• Isotype: Ig
• NCBI Gene Id: 8767
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: RIPK2 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
  Cellular Location: Cytoplasm (Probable).
  Tissue Location: Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This RIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-488 amino acids from the C-terminal region of human RIPK2.
• Uniprot ID: O43353
• Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Protein Accession #: NP_003812.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: RIPK2
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC-P
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Ramos
  
  Western blot analysis of anti-RIPK2 Pab (Cat. # OAAB17272) in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab.
• Image 2: 293
  
  Western blot analysis of RIPK2 (arrow) using rabbit polyclonal RIPK2 Antibody (D474) (Cat.# OAAB17272). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RIPK2 gene.
• Image 3: Mouse cerebellum
  
  Western blot analysis of anti-RIPK2 Pab (Cat. # OAAB17272) in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.