KSR1 Antibody - N-terminal region (Phospho-A7) (OAAB17266)

• Product Number: OAAB17266
• Name: KSR1 Antibody - N-terminal region (Phospho-A7) (OAAB17266)
• Molecular Weight: 102032 Da
• Isotype: Ig
• NCBI Gene Id: 8844
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: KSR, RSU2
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: KSR1 Antibody (N-term A7) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity
• Application Info: IHC-P: 1:50~100
• Immunogen: This KSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KSR1.
• Additional Information: Cellular Location: Cytoplasm. Membrane; Peripheral membrane protein (By similarity). Endoplasmic reticulum membrane. Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery (By similarity)
• Uniprot ID: Q8IVT5
• Protein Name: Kinase suppressor of Ras 1
• Protein Accession #: NP_055053.1
• Gene Symbol: KSR1
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: A375
  
  Western blot analysis of anti-KSR1 Pab (Cat. # OAAB17266) in A375 cell lysate. KSR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 2: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.