B-RAF Antibody - middle region (OAAB17264)

• Product Number: OAAB17264
• Name: B-RAF Antibody - middle region (OAAB17264)
• Molecular Weight: 84 kDa
• Isotype: Ig
• NCBI Gene Id: 673
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 2 mg/ml
• Alias Symbols: NS7, B-raf, BRAF1, RAFB1, B-RAF1
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Description of Target: BRAF, a mebmer of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
  Cellular Location: Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
  Tissue Location: Brain and testis.
• Application Info: IF: 1:10~50
  WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Immunogen: This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from the Central region of human B-RAF.
• Uniprot ID: P15056
• Protein Name: Serine/threonine-protein kinase B-raf
• Sample Type Confirmation:

  BRAF is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_004324.2
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: BRAF
• Predicted Species Reactivity: Human
• Application: WB, IHC-P, IF, FC
• Predicted Homology Based on Immunogen Sequence: Chicken, Mouse
• Image 1: human prostata carcinoma
  
  Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center)(Cat.#OAAB17264), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 2: C2C12 cell
  
  Fluorescent image of C2C12 cell stained with BRAF Antibody (Center)(Cat#OAAB17264).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).BRAF immunoreactivity is localized to Cytoplasm significantly.
• Image 3: 293 cell
  
  Western blot analysis of BRAF Antibody (Center) polyclonal antibody(Cat.#OAAB17264)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
• Image 4: Hela cell
  
  B-RAF Antibody (Center) (Cat. #OAAB17264) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.