YES1 Antibody - N - terminal region (OAAB17236)

• Product Number: OAAB17236
• Name: YES1 Antibody - N - terminal region (OAAB17236)
• Molecular Weight: 60801 Da
• Isotype: Ig
• NCBI Gene Id: 7525
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: Yes, c-yes, HsT441, P61-YES
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis
• Additional Information: Cellular Location: Cell membrane. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytosol. Note=Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway
  Tissue Location: Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This YES1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human YES1.
• Intended Use: YES1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P07947
• Protein Name: Tyrosine-protein kinase Yes
• Sample Type Confirmation:

  YES1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_005424.1
• Gene Symbol: YES1
• Predicted Species Reactivity: Human, Mouse
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Mouse Kidney
  
  Western blot analysis of anti-YES Pab (Cat. # OAAB17236) in mouse kidney tissue lysate. YES (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 2: 293
  
  YES Antibody (K11) (Cat. # OAAB17236) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the YES antibody detected the YES protein (arrow).

  YES1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Image 3: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.