LCK Antibody - N-terminal region (OAAB17227)

• Product Number: OAAB17227
• Name: LCK Antibody - N-terminal region (OAAB17227)
• Molecular Weight: 58001 Da
• Isotype: Ig
• NCBI Gene Id: 3932
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: LSK, YT16, IMD22, p56lck, pp58lck
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: LSK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3'-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LSK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t(1;7)(p34;q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
  FC: 1:10~50
• Immunogen: This LCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human LCK.
• Additional Information: Function: Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T- cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
  Cellular Location: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Present in lipid rafts in an inactive form
  Tissue Location: Expressed specifically in lymphoid cells.
• Uniprot ID: P06239
• Protein Name: Tyrosine-protein kinase Lck
• Protein Accession #: NP_001036236.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: LCK
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC-P, IF, FC
• Image 1: human cancer tissue
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: mouse thymus tissue
  
  Western blot analysis of anti-LCK Pab (Cat. #OAAB17227) in mouse thymus tissue lysate. LCK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 3: SK-BR-3 cell
  
  LCK Antibody (I37) (Cat. #OAAB17227) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the LCK antibody detected the LCK protein (arrow).
• Image 4: A2058 cell
  
  Confocal immunofluorescent analysis of LCK Antibody (N-term)(Cat#OAAB17227) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
• Image 5: A2058 cell
  
  LCK Antibody (N-term) (Cat. #OAAB17227) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.