DOK4 Antibody - C-terminal region (OAAB17206)

• Product Number: OAAB17206
• Name: DOK4 Antibody - C-terminal region (OAAB17206)
• Molecular Weight: 37028 Da
• Isotype: Ig
• NCBI Gene Id: 55715
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: IRS5, IRS-5
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: DOK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation May be involved in the regulation of the immune response induced by T-cells
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This DOK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-258 amino acids from the C-terminal region of human DOK4.
• Additional Information: Tissue Location: Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells
• Uniprot ID: Q8TEW6
• Protein Name: Docking protein 4
• Protein Accession #: NP_060580.2
• Gene Symbol: DOK4
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: 293
  
  Western blot analysis of DOK4 (C-term) (arrow) using DOK4 Antibody (C-term) (Cat.# OAAB17206). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK4 gene (Lane 2) (Origene Technologies).