ROR1 Antibody - N-terminal region (OAAB17186)

• Product Number: OAAB17186
• Name: ROR1 Antibody - N-terminal region (OAAB17186)
• Molecular Weight: 104 kDa
• Isotype: Ig
• NCBI Gene Id: 4919
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Varies by lot. See vial for concentration.
• Alias Symbols: NTRKR1, dJ537F10.1
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Description of Target: ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling. In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells
  Cellular Location: Membrane; Single-pass type I membrane protein. Cell projection, axon
  Tissue Location: Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.
• Application Info: WB: 1:1000
  FC: 1:10~50
• Immunogen: This ROR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human ROR1.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q01973
• Protein Name: Tyrosine-protein kinase transmembrane receptor ROR1
• Protein Accession #: NP_001077061.1
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: ROR1
• Predicted Species Reactivity: Human
• Application: WB, FC
• Image 1: 293
  
  Western blot analysis of ROR1 (arrow) using rabbit polyclonal ROR1 Antibody (N-term) (Cat.# OAAB17186). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2) (Origene Technologies).
• Image 2: NCI-H460, ROR1
  
  Flow cytometric analysis of NCI-H460 cells using ROR1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.