RET Antibody - N-terminal region (OAAB17184)

• Product Number: OAAB17184
• Name: RET Antibody - N-terminal region (OAAB17184)
• Molecular Weight: 124 kDa
• Isotype: Ig
• NCBI Gene Id: 5979
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.65 mg/ml
• Alias Symbols: PTC, MTC1, HSCR1, MEN2A, MEN2B, CDHF12, CDHR16, RET-ELE1
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut- associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell- signaling in the brainstem which induces inhibition of food- intake. Activates MAPK- and AKT-signaling pathways. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein
• Application Info: WB: 1:1,000
  FC: 1:10~50
  Optimal dilutions should be determined by the end user.
• Immunogen: This RET antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-44 amino acids from the N-terminal region of human RET.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P07949
• Protein Name: Proto-oncogene tyrosine-protein kinase receptor Ret
• Protein Accession #: NP_065681.1
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: RET
• Predicted Species Reactivity: Human
• Application: WB, FC, E
• Image 1: MCF7
  
  Western blot analysis of hRET-G28 (Cat. # OAAB17184) in MCF7 cell line lysates (35ug/lane). RET (arrow) was detected using the purified Pab.

  RET is strongly supported by BioGPS gene expression data to be expressed in MCF7

• Image 2: MCF-7
  
  Flow cytometric analysis of MCF-7 cells using RET Antibody (N-term Q28) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.