PDGFRA Antibody - C-terminal region (Phospho-E1063) (OAAB17179)

• Product Number: OAAB17179
• Name: PDGFRA Antibody - C-terminal region (Phospho-E1063) (OAAB17179)
• Molecular Weight: 122670 Da
• Isotype: Ig
• NCBI Gene Id: 5156
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Alias Symbols: CD140A, PDGFR2, PDGFR-2
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Note=The activated receptor is rapidly internalized and degraded
  Tissue Location: Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Immunogen: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.
• Uniprot ID: P16234
• Protein Name: Platelet-derived growth factor receptor alpha
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that PDGFRA is expressed in K562

• Protein Accession #: NP_006197.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: PDGFRA
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC-P, FC
• Predicted Homology Based on Immunogen Sequence: Chicken, Rat
• Image 1: PDGFRA, U251
  
  PDGFRA Antibody (C-term E1063) (Cat. # OAAB17179) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 2: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 3: K562, PDGFRA
  
  Western blot analysis of PDGFRA Antibody (C-term E1063) (Cat.# OAAB17179) in K562 cell line lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.

  There is BioGPS gene expression data showing that PDGFRA is expressed in K562

• Image 4: Mouse Lung, PDGFRA
  
  Western blot analysis of PDGFRA antibody (C-term E1063) (Cat.# OAAB17179) in mouse lung tissue lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.