DDR1 Antibody - N-terminal region (OAAB17175)

• Product Number: OAAB17175
• Name: DDR1 Antibody - N-terminal region (OAAB17175)
• Molecular Weight: 101 kDa
• Isotype: Ig
• NCBI Gene Id: 780
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Varies by lot. See vial for concentration.
• Alias Symbols: CAK, DDR, NEP, HGK2, PTK3, RTK6, TRKE, CD167, EDDR1, MCK10, NTRK4, PTK3A
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. The gene is located on chromosome 6p21.3 in proximity to several HLA class I genes.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
  Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Secreted (Potential).
  Tissue Location: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. {ECO:0000269|PubMed:7845687, ECO:0000269|PubMed:7848919, ECO:0000269|PubMed:8247543}.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:25
• Immunogen: This DDR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-47 amino acids from the N-terminal region of human DDR1.
• Intended Use: DDR1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q08345
• Protein Name: Epithelial discoidin domain-containing receptor 1
• Protein Accession #: NP_001189450.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: DDR1
• Predicted Species Reactivity: Human, Rat
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Mouse
• Image 1: human kidney tissue
  
  DDR1 Antibody (N-term) (Cat. #OAAB17175)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DDR1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: placenta
  
  Western blot analysis of anti-DDR1 Antibody (N-term) Pab (Cat. #OAAB17175) in placenta lysate. DDR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 3: 293 cell
  
  DDR1 Antibody (N-term) (Cat. #OAAB17175) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.