EphB6 Antibody - N-terminal region (Phospho-S45) (OAAB17143)

• Product Number: OAAB17143
• Name: EphB6 Antibody - N-terminal region (Phospho-S45) (OAAB17143)
• Molecular Weight: 110700 Da
• Isotype: Ig
• NCBI Gene Id: 2051
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: HEP
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: EphB6 Antibody (N-term S45) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Immunogen: This EphB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human EphB6.
• Additional Information: Cellular Location: Membrane; Single-pass type I membrane protein
  Tissue Location: Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcoma
• Uniprot ID: O15197
• Protein Name: Ephrin type-B receptor 6
• Protein Accession #: NP_004436.2
• Gene Symbol: EPHB6
• Predicted Species Reactivity: Human
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: MCF-7
  
  Flow cytometric analysis of MCF-7 cells using EphB6 Antibody (N-term S45) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 3: A549
  
  Western blot analysis of anti-EphB6 N-term Pab (Cat. # OAAB17143) in A549 cell lysate. EphB6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.