EphB1 Antibody - C-terminal region (OAAB17138)

• Product Number: OAAB17138
• Name: EphB1 Antibody - C-terminal region (OAAB17138)
• Molecular Weight: 109885 Da
• Isotype: Ig
• NCBI Gene Id: 2047
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ELK, NET, Hek6, EPHT2
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: EphB1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2 In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This EphB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-984 amino acids from the C-terminal region of human EphB1.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, dendrite (By similarity)
  Tissue Location: Preferentially expressed in brain.
• Uniprot ID: P54762
• Protein Name: Ephrin type-B receptor 1
• Protein Accession #: NP_004432.1
• Gene Symbol: EPHB1
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: EphB1
  
  Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d Loss of EphB1 expression in gastric cancer cells.(Provided by Jian-dong Wang,Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine)
• Image 2: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 3: EphB1, Mouse Brain
  
  Western blot analysis of anti-EphB1 Pab (Cat. # OAAB17138) in mouse brain tissue. EphB1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 4: EphB1, T47D
  
  EPHB1 Antibody (H970) (Cat. # OAAB17138) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the EPHB1 antibody detected the EPHB1 protein (arrow).