EphA7 Antibody - C-terminal region (OAAB17136)

• Product Number: OAAB17136
• Name: EphA7 Antibody - C-terminal region (OAAB17136)
• Molecular Weight: 112097 Da
• Isotype: Ig
• NCBI Gene Id: 2045
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: EHK3, EK11, EHK-3, HEK11
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: EphA7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This EphA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human EphA7.
• Additional Information: Cellular Location: Cell membrane (Probable); Single-pass type I membrane protein (Probable)
  Tissue Location: Widely expressed.
• Uniprot ID: Q15375
• Protein Name: Ephrin type-A receptor 7
• Sample Type Confirmation:

  EPHA7 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_004431.1
• Gene Symbol: EPHA7
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: EphA7, NCI-H460
  
  Western blot analysis of anti-EphA7 Pab (Cat. # OAAB17136) in NCI-H460 cell lysate. EphA7 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 3: 293, EphA7
  
  Western blot analysis of EPHA7 (arrow) using EphA7 Antibody (C-term) (Cat.# OAAB17136). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA7 gene (Lane 2) (Origene Technologies).

  EPHA7 is strongly supported by BioGPS gene expression data to be expressed in HEK293