EphA5 Antibody - C-terminal region (OAAB17132)

• Product Number: OAAB17132
• Name: EphA5 Antibody - C-terminal region (OAAB17132)
• Molecular Weight: 114803 Da
• Isotype: Ig
• NCBI Gene Id: 2044
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: EK7, CEK7, EHK1, HEK7, EHK-1, TYRO4
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: EphA5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5 Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity)
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This EphA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 942-972 amino acids from the C-terminal region of human EphA5.
• Additional Information: Cellular Location: Cell membrane (By similarity); Single-pass type I membrane protein (By similarity). Cell projection, axon (By similarity). Cell projection, dendrite
  Tissue Location: Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord
• Uniprot ID: P54756
• Protein Name: Ephrin type-A receptor 5
• Protein Accession #: NP_004430.4
• Gene Symbol: EPHA5
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Mouse Brain
  
  Western blot analysis of anti-EphA5 C-term Pab (Cat. # OAAB17132) in mouse brain tissue. EphA5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 2: EphA5, Jurkat
  
  Western blot analysis of anti-EphA5 Antibody (C-term) (Cat. # OAAB17132) in Jurkat cell line lysates (35ug/lane). EPHA5(arrow) was detected using the purified Pab.
• Image 3: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.