EphA3 Antibody - C-terminal region (OAAB17128)

• Product Number: OAAB17128
• Name: EphA3 Antibody - C-terminal region (OAAB17128)
• Molecular Weight: 110131 Da
• Isotype: Ig
• NCBI Gene Id: 2042
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: EK4, ETK, HEK, ETK1, HEK4, TYRO4
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: EphA3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This EphA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-928 amino acids from the C-terminal region of human EphA3.
• Additional Information: Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein
  Tissue Location: Widely expressed. Highest level in placenta.
• Uniprot ID: P29320
• Protein Name: Ephrin type-A receptor 3
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that EPHA3 is expressed in HEK293

• Protein Accession #: NP_005224.2
• Gene Symbol: EPHA3
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: CHO
  
  Western blot analysis of anti-EphA3 Pab (Cat. # OAAB17128) in CHO cell lysate. EphA3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

  There is BioGPS gene expression data showing that EPHA3 is expressed in HEK293

• Image 2: 293
  
  Western blot analysis of EphA3(arrow) using rabbit polyclonal EphA3 Antibody (C-term) (Cat. # OAAB17128).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2) (Origene Technologies).
• Image 3: Human Placenta
  
  Formalin-fixed and paraffin-embedded human placenta tissue reacted with EphA3 antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.