EphA2 Antibody - N-terminal region (OAAB17126)

• Product Number: OAAB17126
• Name: EphA2 Antibody - N-terminal region (OAAB17126)
• Molecular Weight: 108 kDa
• Isotype: Ig
• NCBI Gene Id: 1969
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 2 mg/ml
• Alias Symbols: ECK, CTPA, ARCC2, CTPP1, CTRCT6
• Product Format: Liquid PBS with 0.09% (w/v) sodium azide
• Description of Target: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single- pass type I membrane protein. Cell junction, focal adhesion Note=Present at regions of cell-cell contacts but also at the leading edge of migrating cells
  Tissue Location: Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g skin, intestine, lung, and ovary
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Immunogen: This EphA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human EphA2.
• Uniprot ID: P29317
• Protein Name: Ephrin type-A receptor 2
• Protein Accession #: NP_004422.2
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: EPHA2
• Predicted Species Reactivity: Monkey
• Application: WB, FC, IHC-P
• Image 1: NCI-H292
  
  Flow cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 2: MCF-7
  
  Western blot analysis of hEPHA2-T45 (Cat. # OAAB17126) in MCF-7 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab
• Image 3: Mouse NIH-3T3
  
  Western blot analysis of hEPHA2-T45 (Cat. # OAAB17126) in mouse NIH-3T3 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab.
• Image 4: 293
  
  Western blot analysis of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45 (Cat. # OAAB17126). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)