EphA1 Antibody - N-terminal region (OAAB17124)

Data Sheet
 
Product Number OAAB17124
Product Page www.avivasysbio.com/epha1-antibody-n-terminal-region-oaab17124.html
Name EphA1 Antibody - N-terminal region (OAAB17124)
Molecular Weight 108127 Da
Isotype Ig
NCBI Gene Id 2041
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols EPH, EPHT, EPHT1
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB17124
Additional Information Precautions: EphA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis
Application Info WB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50
Immunogen This EphA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-60 amino acids from the N-terminal region of human EphA1.
Additional Information Cellular Location: Cell membrane; Single-pass type I membrane protein
Tissue Location: Overexpressed in several carcinomas.
Uniprot ID P21709
Protein Name Ephrin type-A receptor 1
Protein Accession # NP_005223.4
Gene Symbol EPHA1
Predicted Species Reactivity Human
Application WB, IHC, FC
Predicted Homology Based on Immunogen Sequence Human
Image 1
hepatocarcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Image 2
HepG2
Flow cytometric analysis of HepG2 cells using EphA1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Image 3
Hela
Western blot analysis of anti-EphA1 N-term Pab (Cat. # OAAB17124) in HeLa cell lysate. EphA1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
 

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