HIPK3 Antibody - C-terminal region (OAAB17101)

• Product Number: OAAB17101
• Name: HIPK3 Antibody - C-terminal region (OAAB17101)
• Molecular Weight: 133743 Da
• Isotype: Ig
• NCBI Gene Id: 10114
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PKY, YAK1, DYRK6, FIST3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: HIPK3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF- responsive element (OCFRE)
• Application Info: IHC-P: 1:25
  WB: 1:2000
  WB: 1:1000
• Immunogen: This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1127-1156 amino acids from the C-terminal region of human HIPK3.
• Additional Information: Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung
• Uniprot ID: Q9H422
• Protein Name: Homeodomain-interacting protein kinase 3
• Protein Accession #: NP_001041665.1
• Gene Symbol: HIPK3
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: H1299
  
  Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and Flag-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-Flag antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and Flag-sHIPK3.