CrkRS Antibody - N-terminal region (OAAB17095)

• Product Number: OAAB17095
• Name: CrkRS Antibody - N-terminal region (OAAB17095)
• Molecular Weight: 164155 Da
• Isotype: Ig
• NCBI Gene Id: 51755
• Host: Rabbit
• Clonality: Polyclonal
• Alias Symbols: CRK7, CRKR, CRKRS
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
  Cellular Location: Nucleus. Nucleus speckle. Note=Colocalized with nuclear speckles throughout interphase
  Tissue Location: Ubiquitously expressed.
• Application Info: WB: 1:1000
• Immunogen: This CrkRS (CRK7) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CrkRS (CRK7).
• Uniprot ID: Q9NYV4
• Protein Name: Cyclin-dependent kinase 12
• Protein Accession #: NP_055898.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: CDK12
• Predicted Species Reactivity: Human, Mouse
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Rat
• Image 1: Mouse Liver
  
  The anti-CRK7 Pab (Cat. # OAAB17095) is used in Western blot to detect CRK7 in mouse liver tissue lysate.