MAPK12 Antibody - C-terminal region (OAAB17072)

• Product Number: OAAB17072
• Name: MAPK12 Antibody - C-terminal region (OAAB17072)
• Molecular Weight: 41940 Da
• Isotype: Ig
• NCBI Gene Id: 6300
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ERK3, ERK6, ERK-6, SAPK3, PRKM12, SAPK-3, MAPK 12, P38GAMMA
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MAPK12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2 Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma- radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-367 amino acids from the C-terminal region of human MAPK12.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Mitochondrion. Note=Mitochondrial when associated with SH3BP5. In skeletal muscle co-localizes with SNTA1 at the neuromuscular junction and throughout the sarcolemma (By similarity)
  Tissue Location: Highly expressed in skeletal muscle and heart.
• Uniprot ID: P53778
• Protein Name: Mitogen-activated protein kinase 12
• Protein Accession #: NP_002960.2
• Gene Symbol: MAPK12
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Human Skeletal Muscle
  
  Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with the P38 gamma antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 2: Mouse Muscle
  
  Western blot analysis of anti-P38gamma C-term Pab (Cat. # OAAB17072) in mouse tissue lysate. P38gamma (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.