MAPK7 Antibody - C-terminal region (OAAB17061)

Data Sheet
 
Product Number OAAB17061
Product Page www.avivasysbio.com/mapk7-antibody-c-terminal-region-oaab17061.html
Name MAPK7 Antibody - C-terminal region (OAAB17061)
Molecular Weight 88386 Da
Isotype Ig
NCBI Gene Id 5598
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols BMK1, ERK4, ERK5, PRKM7
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB17061
Additional Information Precautions: MAPK7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression
Application Info WB: 1:1000
IHC-P: 1:50~100
Immunogen This MAPK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 776-805 amino acids from the C-terminal region of human MAPK7.
Additional Information Cellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation
Tissue Location: Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver
Uniprot ID Q13164
Protein Name Mitogen-activated protein kinase 7
Protein Accession # NP_002740.2
Gene Symbol MAPK7
Predicted Species Reactivity Human
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human
Image 1
breast carcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Image 2
Human kideny
Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term) (Cat.# OAAB17061), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Image 3
K562
Western blot analysis of anti-ERK5 C-term Pab (Cat. # OAAB17061) in K562 cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.
 

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