Product Number |
OAAB17060 |
Product Page |
www.avivasysbio.com/erk4-antibody-c-terminal-region-oaab17060.html |
Name |
ERK4 Antibody - C-terminal region (OAAB17060) |
Molecular Weight |
65922 Da |
Isotype |
Ig |
NCBI Gene Id |
5596 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
ERK4, ERK-4, PRKM4, p63MAPK, p63-MAPK |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB17060 |
Additional Information |
Precautions: ERK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity) |
Application Info |
WB: 1:1000 IHC-P: 1:10~50 |
Immunogen |
This ERK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-557 amino acids from the C-terminal region of human ERK4. |
Additional Information |
Cellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates to the cytoplasm following interaction with MAPKAPK5 (By similarity) Tissue Location: High expression in heart and brain. |
Uniprot ID |
P31152 |
Protein Name |
Mitogen-activated protein kinase 4 |
Protein Accession # |
NP_002738.2 |
Gene Symbol |
MAPK4 |
Predicted Species Reactivity |
Human, Mouse |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Human; Mouse |
Image 1 | Mouse Brain
| Western blot analysis of anti-ERK4 Pab (Cat. # OAAB17060) in mouse brain tissue lysate. ERK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
| Image 2 | Human lung carcinoma
| Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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