ANXA2 Antibody - N-terminal region (OAAB17056)

• Product Number: OAAB17056
• Name: ANXA2 Antibody - N-terminal region (OAAB17056)
• Molecular Weight: 38604 Da
• Isotype: Ig
• NCBI Gene Id: 302
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: P36, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, PAP-IV, HEL-S-270
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: ANXA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids It binds two calcium ions with high affinity. May be involved in heat-stress response
• Application Info: IHC-P: 1:25
  WB: 1:2000
  WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human ANXA2.
• Additional Information: Cellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism
• Uniprot ID: P07355
• Protein Name: Annexin A2
• Sample Type Confirmation:

  ANXA2 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

• Protein Accession #: NP_001002857.1
• Gene Symbol: ANXA2
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: 293, ANXA2
  
  Western blot analysis of ANXA2 (arrow) using rabbit polyclonal ANXA2 Antibody (N-term) (Cat.# OAAB17056). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.

  ANXA2 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

• Image 2: ANXA2, Mouse Lung
  
  Western blot analysis of anti-ANXA2 Antibody (N-term) (Cat.# OAAB17056) in mouse lung tissue lysates (35ug/lane). ANXA2(arrow) was detected using the purified Pab (1:60 dilution).
• Image 3: 293, ANXA2, MDA-MB231
  
  ANXA2 Antibody (N-term) (Cat. # OAAB17056) western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ANXA2 antibody detected the ANXA2 protein (arrow).
• Image 4: ANXA2, Human lung carcinoma
  
  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (N-term) (Cat.# OAAB17056), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.