Product Number |
OAAB17053 |
Product Page |
www.avivasysbio.com/vrk1-antibody-center-region-oaab17053.html |
Name |
VRK1 Antibody - middle region (OAAB17053) |
Molecular Weight |
45476 Da |
Isotype |
Ig |
NCBI Gene Id |
7443 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
PCH1, PCH1A |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB17053 |
Additional Information |
Precautions: VRK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3 Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm |
Application Info |
WB: 1:2000 |
Immunogen |
This VRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-396 amino acids from the Central region of human VRK1. |
Additional Information |
Cellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle (By similarity). Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis Tissue Location: Widely expressed. Highly expressed in fetal liver, testis and thymus |
Uniprot ID |
Q99986 |
Protein Name |
Serine/threonine-protein kinase VRK1 |
Sample Type Confirmation |
VRK1 is strongly supported by BioGPS gene expression data to be expressed in HEK293, HL60 |
Protein Accession # |
NP_003375.1 |
Gene Symbol |
VRK1 |
Predicted Species Reactivity |
Human, Mouse |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Human; Mouse |
Image 1 | HL-60, Mouse Lung
| The anti-VRK1 Pab (Cat. # OAAB17053) is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2). |
| Image 2 | 293
| Western blot analysis of VRK1 (arrow) using rabbit polyclonal VRK1 Antibody (E381) (Cat. # OAAB17053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK1 gene. VRK1 is strongly supported by BioGPS gene expression data to be expressed in HEK293, HL60 |
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