Rictor Antibody - N-terminal region (OAAB17042)

• Product Number: OAAB17042
• Name: Rictor Antibody - N-terminal region (OAAB17042)
• Molecular Weight: 192218 Da
• Isotype: Ig
• NCBI Gene Id: 253260
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PIA, AVO3, hAVO3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: Rictor Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development
• Application Info: WB: 1:1000
  IHC-P: 1:50
• Immunogen: This Rictor antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 amino acids from the N-terminal region of human Rictor.
• Uniprot ID: Q6R327
• Protein Name: Rapamycin-insensitive companion of mTOR
• Protein Accession #: NP_689969.2
• Gene Symbol: RICTOR
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Ramos
  
  Western blot analysis of Rictor Antibody (N-term) (Cat. # OAAB17042) in Ramos cell line lysates (35ug/lane). RICTOR (arrow) was detected using the purified Pab.
• Image 2: Human lymph
  
  Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term) (Cat.# OAAB17042), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.