STK11 Antibody - N-terminal region (Phospho-I29) (OAAB17017)

• Product Number: OAAB17017
• Name: STK11 Antibody - N-terminal region (Phospho-I29) (OAAB17017)
• Molecular Weight: 48636 Da
• Isotype: Ig
• NCBI Gene Id: 6794
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PJS, LKB1, hLKB1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: STK11 (LKB1) Antibody (N-term I29) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53 Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1 Also acts as a mediator p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways
• Application Info: IHC-P: 1:50~100
  WB: 1:1000
  FC: 1:10~50
  IF: 1:10~50
• Immunogen: This STK11 (LKB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-44 amino acids from the N-terminal region of human STK11 (LKB1).
• Additional Information: Cellular Location: Nucleus. Cytoplasm. Membrane (By similarity). Mitochondrion. Note=A small fraction localizes at membranes (By similarity). Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to mitochondrion during apoptosis
  Tissue Location: Ubiquitously expressed. Strongest expression in testis and fetal liver
• Uniprot ID: Q15831
• Protein Name: Serine/threonine-protein kinase STK11
• Protein Accession #: NP_000446.1
• Gene Symbol: STK11
• Predicted Species Reactivity: Human
• Application: IHC, WB, FC, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: T47D
  
  STK11 Antibody (I29) (Cat. # OAAB17017) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the STK11 antibody detected the STK11 protein (arrow).
• Image 2: 293
  
  Western blot analysis of STK11 (arrow) using rabbit polyclonal STK11 Antibody (Cat.# OAAB17017). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK11 gene (Lane 2) (Origene Technologies).
• Image 3: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 4: NCI-H460
  
  STK11 (LKB1) Antibody (N-term I29) (Cat. # OAAB17017) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 5: Nuclear, ZR-75-1
  
  Confocal immunofluorescent analysis of STK11 (LKB1) Antibody (N-term I29) (Cat# OAAB17017) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).