PHKG2 Antibody - N-terminal region (OAAB17009)

• Product Number: OAAB17009
• Name: PHKG2 Antibody - N-terminal region (OAAB17009)
• Molecular Weight: 46442 Da
• Isotype: Ig
• NCBI Gene Id: 5261
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: GSD9C
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PHKG2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM (By similarity)
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This PHKG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human PHKG2.
• Uniprot ID: P15735
• Protein Name: Phosphorylase b kinase gamma catalytic chain, testis/liver isoform
• Sample Type Confirmation:

  PHKG2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_000285.1
• Gene Symbol: PHKG2
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: Mouse Kidney
  
  The anti-PHKG2 Pab (Cat. # OAAB17009) is used in Western blot to detect PHKG2 in mouse kidney tissue lysate.
• Image 3: 293
  
  Western blot analysis of PHKG2 (arrow) using PHKG2 Antibody (N-term) (Cat.# OAAB17009).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHKG2 gene (Lane 2) (Origene Technologies).

  PHKG2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Image 4: Hela
  
  PHKG2 Antibody (E56) (Cat. # OAAB17009) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).