SEPSH2 Antibody - middle region (OAAB16961)

• Product Number: OAAB16961
• Name: SEPSH2 Antibody - middle region (OAAB16961)
• Molecular Weight: 47 kDa
• Isotype: Ig
• NCBI Gene Id: 22928
• Host: Rabbit
• Clonality: Polyclonal
• Purity: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Alias Symbols: SPS2
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Reference: Tamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 101(46):16162-16167 (2004).
  Lescure, A., et al., J. Biol. Chem. 274(53):38147-38154 (1999).
  Guimaraes, M.J., et al., Proc. Natl. Acad. Sci. U.S.A. 93(26):15086-15091 (1996).
  Guimaraes, M.J., et al., Development 121(10):3335-3346 (1995).
• Description of Target: This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Synthesizes selenophosphate from selenide and ATP.
• Application Info: WB: 1:1,000
  IHC-P: 1:50~100
  Optimal dilutions should be determined by the end user.
• Immunogen: This SEPSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-201 amino acids from the Central region of human SEPSH2.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q99611
• Protein Name: Selenide, water dikinase 2
• Sample Type Confirmation:

  SEPHS2 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_036380.2
• Gene Symbol: SEPHS2
• Predicted Species Reactivity: Human
• Application: WB, IHC-P, E
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HepG2
  
  Western blot analysis of anti-SEPSH2 Pab(Cat. # OAAB16961) in HepG2 cell line lysate (35ug/lane). SEPSH2(arrow) was detected using the purified Pab (1:50 dilution).

  SEPHS2 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Image 2: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.