RFK Antibody - N-terminal region (OAAB16955)

• Product Number: OAAB16955
• Name: RFK Antibody - N-terminal region (OAAB16955)
• Molecular Weight: 17623 Da
• Isotype: Ig
• NCBI Gene Id: 55312
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: RIFK
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: RFK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN)
• Application Info: IHC-P: 1:50~100
  WB: 1:1000
  FC: 1:10~50
• Immunogen: This RFK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RFK.
• Additional Information: Cellular Location: Cytoplasm (By similarity).
  Tissue Location: Detected in brain, placenta and urinary bladder
• Uniprot ID: Q969G6
• Protein Name: Riboflavin kinase
• Sample Type Confirmation:

  RFK is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_060809.3
• Gene Symbol: RFK
• Predicted Species Reactivity: Human
• Application: IHC, WB, FC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: 293
  
  Western blot analysis of RFK (arrow) using rabbit polyclonal RFK Antibody (C15) (Cat. # OAAB16955). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFK gene.

  RFK is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Image 2: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 3: 293
  
  RFK Antibody (N-term) (Cat. # OAAB16955) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.