Pyruvate Kinase Antibody - C-terminal region (Phospho-N491) (OAAB16943)

Data Sheet
 
Product Number OAAB16943
Product Page www.avivasysbio.com/pyruvate-kinase-pkm2-antibody-c-terminal-region-n491-oaab16943.html
Name Pyruvate Kinase Antibody - C-terminal region (Phospho-N491) (OAAB16943)
Molecular Weight 57937 Da
Isotype Ig
NCBI Gene Id 5315
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols PK3, TCB, p58, OIP3, PKM2, CTHBP, THBP1, HEL-S-30
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16943
Additional Information Precautions: Pyruvate Kinase (PKM2) Antibody (C-term N491) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival
Application Info WB: 1:1000
Immunogen This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
Additional Information Cellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity
Tissue Location: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells
Uniprot ID P14618
Protein Name Pyruvate kinase isozymes M1/M2
Protein Accession # NP_001193725.1
Gene Symbol PKM
Predicted Species Reactivity Human, Mouse
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human; Mouse
Image 1
Mouse Brain, PKM2
Western blot analysis of hPKM2-N491 (Cat.# OAAB16943) in A2058 cell line(lane 1) and mouse brain tissue(lane 2) lysates (35ug/lane). PKM2 (arrow) was detected using the purified Pab.
Image 2
human cancer
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
 

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