Product Number |
OAAB16806 |
Product Page |
www.avivasysbio.com/map4k2-antibody-middle-region-oaab16806.html |
Name |
MAP4K2 Antibody - middle region (OAAB16806) |
Molecular Weight |
91556 Da |
Isotype |
Ig |
NCBI Gene Id |
5871 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
GCK, BL44, RAB8IP |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB16806 |
Additional Information |
Precautions: Germinal Center Kinase (GCK/MAP4K2) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3 May play a role in the regulation of vesicle targeting or fusion regulation of vesicle targeting or fusion |
Application Info |
WB: 1:1000 IHC-P: 1:50~100 |
Immunogen |
This Germinal Center Kinase (GCK/MAP4K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-319 amino acids from the Central region of human Germinal Center Kinase (GCK/MAP4K2). |
Additional Information |
Cellular Location: Cytoplasm (By similarity). Basolateral cell membrane; Peripheral membrane protein (By similarity). Golgi apparatus membrane; Peripheral membrane protein (By similarity) Tissue Location: Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined |
Uniprot ID |
Q12851 |
Protein Name |
Mitogen-activated protein kinase kinase kinase kinase 2 |
Protein Accession # |
NP_004570.2 |
Gene Symbol |
MAP4K2 |
Predicted Species Reactivity |
Human, Mouse |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Human; Mouse |
Image 1 | Mouse skeletal Muscle
| The anti-MAP4K2 Pab (Cat. # OAAB16806) is used in Western blot to detect MAP4K2 in mouse skeletal tissue lysate. |
| Image 2 | hepatocarcinoma
| Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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