MAP4K2 Antibody - middle region (OAAB16806)

Data Sheet
 
Product Number OAAB16806
Product Page www.avivasysbio.com/map4k2-antibody-middle-region-oaab16806.html
Name MAP4K2 Antibody - middle region (OAAB16806)
Molecular Weight 91556 Da
Isotype Ig
NCBI Gene Id 5871
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols GCK, BL44, RAB8IP
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16806
Additional Information Precautions: Germinal Center Kinase (GCK/MAP4K2) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3 May play a role in the regulation of vesicle targeting or fusion regulation of vesicle targeting or fusion
Application Info WB: 1:1000
IHC-P: 1:50~100
Immunogen This Germinal Center Kinase (GCK/MAP4K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-319 amino acids from the Central region of human Germinal Center Kinase (GCK/MAP4K2).
Additional Information Cellular Location: Cytoplasm (By similarity). Basolateral cell membrane; Peripheral membrane protein (By similarity). Golgi apparatus membrane; Peripheral membrane protein (By similarity)
Tissue Location: Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined
Uniprot ID Q12851
Protein Name Mitogen-activated protein kinase kinase kinase kinase 2
Protein Accession # NP_004570.2
Gene Symbol MAP4K2
Predicted Species Reactivity Human, Mouse
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human; Mouse
Image 1
Mouse skeletal Muscle
The anti-MAP4K2 Pab (Cat. # OAAB16806) is used in Western blot to detect MAP4K2 in mouse skeletal tissue lysate.
Image 2
hepatocarcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
 

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