PRKAB1 Antibody - N-terminal region (OAAB16785)

• Product Number: OAAB16785
• Name: PRKAB1 Antibody - N-terminal region (OAAB16785)
• Molecular Weight: 30382 Da
• Isotype: Ig
• NCBI Gene Id: 5564
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: AMPK, HAMPKb
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: AMPK beta (PRKAB1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3)
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This AMPK beta (PRKAB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 amino acids from the N-terminal region of human AMPK beta (PRKAB1).
• Uniprot ID: Q9Y478
• Protein Name: 5'-AMP-activated protein kinase subunit beta-1
• Sample Type Confirmation:

  PRKAB1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_006244.2
• Gene Symbol: PRKAB1
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Jurkat, Mouse Spleen
  
  The anti-PRKAB1 Pab (Cat. # OAAB16785) is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).

  PRKAB1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Image 2: Mouse Heart
  
  Western blot analysis of anti-PRKAB1 Antibody (N-term) (Cat.# OAAB16785) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab.
• Image 3: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.