Product Number |
OAAB16771 |
Product Page |
www.avivasysbio.com/lats1-antibody-n-terminal-region-oaab16771.html |
Name |
LATS1 Antibody - N-terminal region (OAAB16771) |
Molecular Weight |
126870 Da |
Isotype |
Ig |
NCBI Gene Id |
9113 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
wts, WARTS |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB16771 |
Additional Information |
Precautions: LATS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function |
Application Info |
WB: 1:1000 IHC-P: 1:50~100 |
Immunogen |
This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LATS1. |
Additional Information |
Cellular Location: Cytoplasm, cytoskeleton, centrosome. Note=Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis Tissue Location: Expressed in all adult tissues examined except for lung and kidney |
Uniprot ID |
O95835 |
Protein Name |
Serine/threonine-protein kinase LATS1 |
Sample Type Confirmation |
LATS1 is strongly supported by BioGPS gene expression data to be expressed in K562 |
Protein Accession # |
NP_001257448.1 |
Gene Symbol |
LATS1 |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Human; Mouse; Rat |
Image 1 | K562
| Western blot analysis of anti-LATS1 Antibody (N-term) (Cat.# OAAB16771) in K562 cell line lysates (35ug/lane). LATS1 (arrow) was detected using the purified Pab. LATS1 is strongly supported by BioGPS gene expression data to be expressed in K562 |
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Image 2 | hepatocarcinoma
| Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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