LATS1 Antibody - N-terminal region (OAAB16771)

Data Sheet
 
Product Number OAAB16771
Product Page www.avivasysbio.com/lats1-antibody-n-terminal-region-oaab16771.html
Name LATS1 Antibody - N-terminal region (OAAB16771)
Molecular Weight 126870 Da
Isotype Ig
NCBI Gene Id 9113
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols wts, WARTS
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16771
Additional Information Precautions: LATS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function
Application Info WB: 1:1000
IHC-P: 1:50~100
Immunogen This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LATS1.
Additional Information Cellular Location: Cytoplasm, cytoskeleton, centrosome. Note=Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis
Tissue Location: Expressed in all adult tissues examined except for lung and kidney
Uniprot ID O95835
Protein Name Serine/threonine-protein kinase LATS1
Sample Type Confirmation

LATS1 is strongly supported by BioGPS gene expression data to be expressed in K562

Protein Accession # NP_001257448.1
Gene Symbol LATS1
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human; Mouse; Rat
Image 1
K562
Western blot analysis of anti-LATS1 Antibody (N-term) (Cat.# OAAB16771) in K562 cell line lysates (35ug/lane). LATS1 (arrow) was detected using the purified Pab.

LATS1 is strongly supported by BioGPS gene expression data to be expressed in K562

Image 2
hepatocarcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
 

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