Product Number |
OAAB16759 |
Product Page |
www.avivasysbio.com/pkc-nu-antibody-c-terminal-region-oaab16759.html |
Name |
PKC nu Antibody - C-terminal region (OAAB16759) |
Molecular Weight |
100471 Da |
Isotype |
Ig |
NCBI Gene Id |
23683 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
EPK2, PKD3, PRKCN, PKC-NU, nPKC-NU |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB16759 |
Additional Information |
Precautions: PKC nu Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity) |
Application Info |
WB: 1:1000 IHC-P: 1:50~100 |
Immunogen |
This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 860-890 amino acids from the C-terminal region of human PKC nu. |
Additional Information |
Cellular Location: Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation Tissue Location: Ubiquitous. |
Uniprot ID |
O94806 |
Protein Name |
Serine/threonine-protein kinase D3 |
Sample Type Confirmation |
PRKD3 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460 |
Protein Accession # |
NP_005804.1 |
Gene Symbol |
PRKD3 |
Predicted Species Reactivity |
Human |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Human |
Image 1 | NCI-H460 cell
| Western blot analysis of anti-PKCnu C-term Pab (Cat. #OAAB16759) in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
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Image 2 | Panc-1 cell
| Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (AP7025a) and C-term (OAAB16759) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using AP7025a and OAAB16759 antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles. |
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Image 3 | human cancer tissue
| Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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