PKC nu Antibody - C-terminal region (OAAB16759)

Data Sheet
 
Product Number OAAB16759
Product Page www.avivasysbio.com/pkc-nu-antibody-c-terminal-region-oaab16759.html
Name PKC nu Antibody - C-terminal region (OAAB16759)
Molecular Weight 100471 Da
Isotype Ig
NCBI Gene Id 23683
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols EPK2, PKD3, PRKCN, PKC-NU, nPKC-NU
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Description of Target Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16759
Additional Information Precautions: PKC nu Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity)
Application Info WB: 1:1000
IHC-P: 1:50~100
Immunogen This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 860-890 amino acids from the C-terminal region of human PKC nu.
Additional Information Cellular Location: Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation
Tissue Location: Ubiquitous.
Uniprot ID O94806
Protein Name Serine/threonine-protein kinase D3
Sample Type Confirmation

PRKD3 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

Protein Accession # NP_005804.1
Gene Symbol PRKD3
Predicted Species Reactivity Human
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human
Image 1
NCI-H460 cell
Western blot analysis of anti-PKCnu C-term Pab (Cat. #OAAB16759) in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Image 2
Panc-1 cell
Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (AP7025a) and C-term (OAAB16759) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using AP7025a and OAAB16759 antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.
Image 3
human cancer tissue
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
 

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