SCYL2 Antibody - C-terminal region (OAAB16757)

• Product Number: OAAB16757
• Name: SCYL2 Antibody - C-terminal region (OAAB16757)
• Molecular Weight: 103709 Da
• Isotype: Ig
• NCBI Gene Id: 55681
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: AMC4, AMCNACC, CVAK104
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: SCYL2 (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system
• Application Info: IHC-P: 1:10~50
• Immunogen: This SCYL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-672 amino acids from the C-terminal region of human SCYL2.
• Additional Information: Cellular Location: Cytoplasm, perinuclear region. Cytoplasmic vesicle, clathrin-coated vesicle. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Note=According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans- Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and
• Uniprot ID: Q6P3W7
• Protein Name: SCY1-like protein 2
• Protein Accession #: NP_060458.3
• Gene Symbol: SCYL2
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Mouse Brain, T47D
  
  Western blot analysis of anti-SCYL2 Pab (OAAB16757) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane). SCYL2(arrow) was detected using the purified Pab (1:60 dilution).
• Image 2: Human Brain
  
  Formalin-fixed and paraffin-embedded human brain tissue reacted with SCYL2 (C-term) (Cat.# OAAB16757), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.