PKC iota Antibody - N-terminal region (OAAB16754)

• Product Number: OAAB16754
• Name: PKC iota Antibody - N-terminal region (OAAB16754)
• Molecular Weight: 68262 Da
• Isotype: Ig
• NCBI Gene Id: 5584
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PKCI, DXS1179E, nPKC-iota
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non- small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis
• Additional Information: Cellular Location: Cytoplasm. Membrane. Endosome. Nucleus. Note=Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A
  Tissue Location: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This PKC iota antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-207 amino acids from the N-terminal region of human PKC iota.
• Intended Use: PKC iota Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P41743
• Protein Name: Protein kinase C iota type
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that PRKCI is expressed in HEK293, Hela

• Protein Accession #: NP_002731.4
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: PRKCI
• Predicted Species Reactivity: Human
• Application: WB, IHC-FFPE
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: 293
  
  Western blot analysis of PRKCI (arrow) using rabbit polyclonal PKC iota Antibody (N-term) (Cat.# OAAB16754). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2) (Origene Technologies).

  There is BioGPS gene expression data showing that PRKCI is expressed in HEK293, Hela

• Image 2: Hela
  
  Western blot analysis of hPKC-iota-C181 (Cat.# OAAB16754) in Hela cell line lysates (35ug/lane).PKC (arrow) was detected using the purified Pab.
• Image 3: Human hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.