PKC beta2 Antibody - C-terminal region (OAAB16748)

• Product Number: OAAB16748
• Name: PKC beta2 Antibody - C-terminal region (OAAB16748)
• Molecular Weight: 76869 Da
• Isotype: Ig
• NCBI Gene Id: 5579
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PKCB, PRKCB1, PRKCB2, PKCI(2), PKCbeta, PKC-beta
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PKC beta2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1- MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity)
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This PKC beta2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 642-673 amino acids from the C-terminal region of human PKC beta2.
• Additional Information: Cellular Location: Cytoplasm (By similarity). Nucleus. Membrane; Peripheral membrane protein (By similarity)
• Uniprot ID: P05771
• Protein Name: Protein kinase C beta type
• Sample Type Confirmation:

  PRKCB is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_002729.2
• Gene Symbol: PRKCB
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Image 1: Jurkat, Mouse Brain
  
  The anti-PKC beta2 Pab (Cat. # OAAB16748) is used in Western blot to detect PKC beta2 in Jurkat cell lysate (lane 1) and mouse brain tissue lysate (lane 2).

  PRKCB is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Image 2: Human Brain
  
  Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.