XPO1 Antibody - C-terminal region (OAAB16714)

• Product Number: OAAB16714
• Name: XPO1 Antibody - C-terminal region (OAAB16714)
• Molecular Weight: 123386 Da
• Isotype: Ig
• NCBI Gene Id: 7514
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: emb, CRM1, exp1, CRM-1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: XPO1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization
• Application Info: IHC-P: 1:50~100
  FC: 1:10~50
• Immunogen: This XPO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 817-846 amino acids from the C-terminal region of human XPO1.
• Additional Information: Cellular Location: Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Note=Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm
  Tissue Location: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney
• Uniprot ID: O14980
• Protein Name: Exportin-1
• Protein Accession #: NP_003391.1
• Gene Symbol: XPO1
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Image 1: Human Brain
  
  Formalin-fixed and paraffin-embedded human brain tissue reacted with XPO1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 2: WiDr
  
  Flow cytometric analysis of widr cells using XPO1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 3: Mouse bladder
  
  Western blot analysis of XPO1 antibody (C-term) (Cat. # OAAB16714) in mouse bladder tissue lysates (35ug/lane). XPO1 (arrow) was detected using the purified Pab.