NURR1 Antibody - N-terminal region (OAAB16706)

• Product Number: OAAB16706
• Name: NURR1 Antibody - N-terminal region (OAAB16706)
• Molecular Weight: 66591 Da
• Isotype: Ig
• NCBI Gene Id: 4929
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: NOT, RNR1, HZF-3, NURR1, TINUR
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Parkinson's disease (PD) is a multifactorial disease that appears to arise from the effects of both genetic and environmental influences. The known genetic factors include multiple genes that have been identified in related parkinsonian syndromes, as well as alpha-synuclein. Genes associated with either PD or Parkinson-related disorders include parkin, DJ-1, ubiquitin C-terminal hydrolase isozyme L1 (UCH-L1), nuclear receptor-related factor 1 (NURR1), and alpha-synuclein. Nurr1 is a transcription factor that is expressed in the embryonic ventral midbrain and is critical for the development of dopamine (DA) neurons. It belongs to the conserved family of nuclear receptors but lacks an identified ligand and is therefore referred to as an orphan receptor. RXR ligands can promote the survival of DA neurons via a process that depends on Nurr1-RXR heterodimers. In developing DA cells, Nurr1 is required for the expression of several genes important for DA synthesis and function. Nurr1 is also important for the maintenance of adult DA neurons.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (By similarity).
  Cellular Location: Cytoplasm. Nucleus. Note=Mostly nuclear; oxidative stress promotes cytoplasmic localization.
  Tissue Location Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
  
• Application Info: IHC-P: 1:50~100
  IF: 1:10~50
• Immunogen: This NURR1 (NR4A2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human NURR1 (NR4A2).
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
  
• Uniprot ID: P43354
• Protein Name: Nuclear receptor subfamily 4 group A member 2
• Protein Accession #: NP_006177.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: NR4A2
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC-P, IF
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat
• Image 1: Hela cell
  
  NR4A2 Antibody (N-term) (Cat. #OAAB16706) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR4A2 antibody detected the NR4A2 protein (arrow).
• Image 2: mouse brain tissue
  
  Western blot analysis of anti-NURR1(NR4A2) Pab (Cat. #OAAB16706) in mouse brain tissue lysate. NURR1(NR4A2) (arrow) was detected using the purified Pab.
• Image 3: Hela cell
  
  Confocal immunofluorescent analysis of NURR1 (NR4A2) Antibody (N-term) (Cat#OAAB16706) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
• Image 4: human brain tissue
  
  Formalin-fixed and paraffin-embedded human brain tissue reacted with NURR1 (NR4A2) antibody (N-term)(Cat#OAAB16706), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.