Parkin Antibody - N-terminal region (OAAB16691)

Data Sheet
 
Product Number OAAB16691
Product Page www.avivasysbio.com/parkin-antibody-n-terminal-region-oaab16691.html
Name Parkin Antibody - N-terminal region (OAAB16691)
Molecular Weight 51641 Da
Isotype Ig
NCBI Gene Id 5071
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols PDJ, AR-JP, LPRS2, PARK2
Product Format Liquid. PBS with 0.09% (W/V) sodium azide.
Description of Target Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known; however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16691
Additional Information Function: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'- linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria. Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in role in regulation of neuron death. Limits the production of reactive oxygen species (ROS). Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin-E during neuronal apoptosis. May represent a tumor suppressor gene

Cellular Location: Cytoplasm, cytosol. Nucleus. Endoplasmic reticulum. Mitochondrion. Note=Mainly localizes in the cytosol Co-localizes with SYT11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. Relocates to dysfunctional mitochondria that have lost the mitochondrial membrane potential; recruitment to mitochondria is PINK1-dependent
Tissue Location: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis. Found in serum (at protein level)
Application Info WB: 1:250~1:1000
FC: 1:10~50
IF: 1:10~50
IHC-P: 1:10~50
Immunogen This Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human Parkin.
Intended Use Parkin Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Uniprot ID O60260
Protein Name E3 ubiquitin-protein ligase parkin
Sample Type Confirmation

PARK2 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

Protein Accession # NP_004553.2
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Gene Symbol PARK2
Predicted Species Reactivity Human
Application WB, FC, IF, IHC-P
Image 1
NCI-H460 cell
Western blot analysis of anti-PARK2 (Parkin) Antibody (N-term) (Cat.#OAAB16691) in NCI-H460 cell line lysates (35ug/lane). PARK2 (Parkin)(arrow) was detected using the purified Pab.
Image 2
human brain tissue
Parkin Antibody (N-term) (OAAB16691)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parkin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Image 3
NCI-H460 cell
Confocal immunofluorescent analysis of Parkin Antibody (N-term)(Cat#OAAB16691) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Image 4
NCI-H460 cell
Parkin Antibody (N-term) (Cat. #OAAB16691) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

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