GPC3 Antibody - middle region (OAAB16663)

• Product Number: OAAB16663
• Name: GPC3 Antibody - middle region (OAAB16663)
• Molecular Weight: 65563 Da
• Isotype: Ig
• NCBI Gene Id: 2719
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition
• Additional Information: Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side (By similarity)
  Tissue Location: Highly expressed in lung, liver and kidney.
• Application Info: WB: 1:1000
  IF: Tested
• Immunogen: This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the Central region of human Glypican 3 (GPC3).
• Intended Use: Glypican 3 (GPC3) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P51654
• Protein Name: Glypican-3
• Sample Type Confirmation:

  GPC3 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_004475.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: GPC3
• Predicted Species Reactivity: Human, Mouse
• Application: IF, WB
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: HepG2
  
  Immunofluorecence staining of anti-GPC3 Pab (cat# OAAB16663) on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.
• Image 2: HepG2
  
  GPC3 Antibody?C349? (cat# OAAB16663) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

  GPC3 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Image 3: Mouse Stomach
  
  GPC3 Antibody?C349? (cat# OAAB16663) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).