SOST Antibody - N-terminal region (OAAB16592)

• Product Number: OAAB16592
• Name: SOST Antibody - N-terminal region (OAAB16592)
• Molecular Weight: 24031 Da
• Isotype: Ig
• NCBI Gene Id: 50964
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: CDD, VBCH, DAND6, SOST1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: SOST Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation
• Application Info: WB: 1:2000
  IHC-P: 1:25
  WB: 1:1000
• Immunogen: This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.
• Additional Information: Cellular Location: Secreted, extracellular space, extracellular matrix
  Tissue Location: Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level)
• Uniprot ID: Q9BQB4
• Protein Name: Sclerostin
• Protein Accession #: NP_079513.1
• Gene Symbol: SOST
• Predicted Species Reactivity: Human, Mouse, Cow
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse
• Image 1: Human breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST antibody (N-term) (Cat.# OAAB16592), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 2: NCI-H292
  
  SOST Antibody (N-term) (Cat. # OAAB16592) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SOST antibody detected the SOST protein (arrow).
• Image 3: 293
  
  Western blot analysis of SOST (arrow) using rabbit polyclonal SOST Antibody (N-term) (Cat. # OAAB16592). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SOST gene.
• Image 4: Mouse Liver
  
  Western blot analysis of anti-SOST Antibody (N-term) Pab (Cat.# OAAB16592) in mouse liver tissue lysates (35ug/lane). SOST (arrow) was detected using the purified Pab (1:60 dilution).