SIRT6 Antibody - C-terminal region (OAAB16579)

• Product Number: OAAB16579
• Name: SIRT6 Antibody - C-terminal region (OAAB16579)
• Molecular Weight: 39119 Da
• Isotype: Ig
• NCBI Gene Id: 51548
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: SIR2L6
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate
• Additional Information: Cellular Location: Nucleus, nucleoplasm. Note=Predominantly nuclear. Associated with telomeric heterochromatin regions
• Application Info: WB: 1:500
  IHC-P: 1:50~100
  WB: 1:1000
• Immunogen: This SIRT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from the C-terminal region of human SIRT6.
• Intended Use: SIRT6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q8N6T7
• Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Accession #: NP_001180214.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: SIRT6
• Predicted Species Reactivity: Human, Mouse
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 2: Mouse Stomach
  
  Western blot analysis of SIRT6 Antibody (C-term) (Cat.# OAAB16579) in mouse stomach tissue lysates (35ug/lane). SIRT6 (arrow) was detected using the purified Pab.