NOTCH3 Antibody - C-terminal region (OAAB16558)

• Product Number: OAAB16558
• Name: NOTCH3 Antibody - C-terminal region (OAAB16558)
• Molecular Weight: 244 kDa
• Isotype: Ig
• NCBI Gene Id: 4854
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Alias Symbols: IMF2, LMNS, CASIL, CADASIL, CADASIL1
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus Affects the implementation of differentiation, proliferation and apoptotic programs.
  Cellular Location: Cell membrane; Single-pass type I membrane protein
  Tissue Location: Ubiquitously expressed in fetal and adult tissues
• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:50~100
• Immunogen: This NOTCH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2291-2321 amino acids from the C-terminal region of human NOTCH3.
• Intended Use: NOTCH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q9UM47
• Protein Name: Neurogenic locus notch homolog protein 3
• Protein Accession #: NP_000426.2
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Tested Species Reactivity: Mouse
• Gene Symbol: NOTCH3
• Predicted Species Reactivity: Human
• Application: WB, IF, IHC-P
• Image 1: Mouse NIH-3T3
  
  NOTCH3-Q2306 (Cat. # OAAB16558) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).
• Image 2: HepG2, Nuclear
  
  Confocal immunofluorescent analysis of NOTCH3 Antibody (C-term) (Cat# OAAB16558) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).