MMP12 Antibody - C-terminal region (OAAB16539)

• Product Number: OAAB16539
• Name: MMP12 Antibody - C-terminal region (OAAB16539)
• Molecular Weight: 54002 Da
• Isotype: Ig
• NCBI Gene Id: 4321
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ME, HME, MME, MMP-12
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MMP12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3
• Application Info: IHC-P: 1:25
  WB: 1:2000
  WB: 1:1000
  IF: 1:10~50
• Immunogen: This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
• Additional Information: Cellular Location: Secreted, extracellular space, extracellular matrix (Probable)
  Tissue Location: Found in alveolar macrophages but not in peripheral blood monocytes
• Uniprot ID: P39900
• Protein Name: Macrophage metalloelastase
• Protein Accession #: NP_002417.2
• Gene Symbol: MMP12
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC, IF
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: 293
  
  Western blot analysis of anti-hMMP12-R406 Pab (Cat. # OAAB16539) pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. MMP12(arrow) was detected using the purified Pab (1:60 dilution) ;
• Image 2: Mouse Kidney
  
  Western blot analysis of anti-hMMP12-R406 Pab (Cat. # OAAB16539) pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates. MMP12(arrow) was detected using the purified Pab (1:60 dilution).
• Image 3: Human breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 4: 293, Nuclear
  
  293, Nuclear