AF9 Antibody - C-terminal region (Phospho-K486) (OAAB16532)

• Product Number: OAAB16532
• Name: AF9 Antibody - C-terminal region (Phospho-K486) (OAAB16532)
• Molecular Weight: 63351 Da
• Isotype: Ig
• NCBI Gene Id: 4300
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: AF9, YEATS3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: AF9 (MLLT3) Antibody (C-term K486) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This AF9 (MLLT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-502 amino acids from the C-terminal region of human AF9 (MLLT3).
• Additional Information: Cellular Location: Nucleus (By similarity).
• Uniprot ID: P42568
• Protein Name: Protein AF-9
• Sample Type Confirmation:

  MLLT3 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_004520.2
• Gene Symbol: MLLT3
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: 293
  
  Western blot analysis of AF9 (MLLT3) Antibody (C-term K486) (Cat.# OAAB16532) in 293 cell line lysates (35ug/lane). MLLT3(arrow) was detected using the purified Pab.

  MLLT3 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Image 2: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.