MAGED1 Antibody - middle region (OAAB16522)

• Product Number: OAAB16522
• Name: MAGED1 Antibody - middle region (OAAB16522)
• Molecular Weight: 86161 Da
• Isotype: Ig
• NCBI Gene Id: 9500
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: NRAGE, DLXIN-1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MAGED1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds NGFR/p75NTR and antagonizes its association with NTRK1/TrkA, inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate TP53/p53 transcriptional activity and inhibit cell proliferation Enhances TP53 phosphorylation and accumulation. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This MAGED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-456 amino acids from the Central region of human MAGED1.
• Additional Information: Cellular Location: Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Note=Expression shifts from the cytoplasm to the plasma membrane upon stimulation with NGF (By similarity)
  Tissue Location: Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed
• Uniprot ID: Q9Y5V3
• Protein Name: Melanoma-associated antigen D1
• Protein Accession #: NP_001005332.1
• Gene Symbol: MAGED1
• Predicted Species Reactivity: Human, Pig
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Pig
• Image 1: A549
  
  The anti-MAGED1 Ctr Antibody (Cat.# OAAB16522) is used in Western blot to detect MAGED1 in A549 lysate.
• Image 2: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.